2. Propolis Description
Propolis is a resinous yellow-brown to dark brown substance collected by worker honey bees from the growing parts of trees and shrubs (eg., leaf buds, trunk wounds). The bees pack the propolis on their hind legs, and carry it back to their colony, where it is combined with beeswax and used by worker "hive" bees as a sealant and sterilant in the colony nest. The uses take advantage of the antibacterial and antifungal effects of propolis in protecting the colony against disease.
Propolis has also been shown to kill Bacillus larvae, the most important bacterial disease of bees (Mlagan and Sulimanovic, 1982).
Propolis changes consistency with temperature. At temperatures below 150C it is hard and brittle, but becomes more pliable and sticky at higher temperatures (25-450C). Propolis generally melts at 60-700C, although some samples have been found to have a melting point as high as 1000C (Krell, 1998). Propolis is collected by commercial beekeepers, either by scraping the substance from wooden hive parts, or by using specially constructed collection mats. The raw product undergoes secondary processing to remove beeswax and other impurities before being used in a variety of natural health care products (eg., lozenges, tinctures, ointments, drinks).
3. History of Use
Propolis has been used by man since early times, for various purposes, and especially as a medicine because of its antimicrobial properties (Crane, 1997). Ancient Greek texts refer to the substance as a " cure for bruises and suppurating sore", and in Rome propolis was used by physicians in making poultices. The Hebrew word for propolis is tzori, and the therapeutic properties of tzori are mentioned throughout the Old Testament. Records from 12th century Europe describe medical preparations using propolis for the treatment of mouth and throat infections, and dental cares (Krell, 1996).
One of the non-medicinal uses of propolis is as a varnish, and it has been suggested that the special properties of Stradivarius violins may be partly due to the type of propolis used, although the claim cannot be substantiated.
At least 180 different compounds have been identified so far in propolis. A list of the major chemicals occurring in propolis is given in the following table (Krell, 1998):
|Class of Compound||Group of Components||Amount|
|Resins||flavonoids, phenolic acids and esters||45-55%>|
|Waxes and Fatty Acids||beeswax and plant origin||25-35%|
|Pollen||proteins (16 free amino acids >1%)
arginine and proline together 46% of total
|Other Organics and Minerals||14 trace minerals, iron and zinc most common;
ketones, lactones, quinones, steroids, benzoic acid, vitamins, sugars
The most Important pharmacologically active constituents in propolis are the flavones, flavonols, and flavanones (collectively called flavonoids), and various phenolics and aromatics. Flavonoids play a major role in plant pigmentation. However, the flavonoids present in propolis are different in composition to those normally found in plants, since propolis flavonoids are not glycosides (ie, they do not have sugar molecules attached to their chemical structure). The majority of flavonoids found in plants are glycosides.
Flavonoids are thought to account for much of the biological activity in propolis (Grange and Davey, 1990), although other phenolic compounds are also involved. At least 38 flavonoids have been found in propolis, including galangin, kaempferol, quercetin, pinocembrin, pinostrobin and pinobanksin. (Schmidt and Buchmann, 1992).
Some of the other phenolics include cinnamic alcohol, cinnamic acid, vanillin, benzyl alcohol, benzoic acid, and caffeic and ferulic acid. The chemical composition of propolis is highly variable because of the broad range of plants visited by honey bees when collecting the substance. Crane (1990) identifies at least 67 species from which honey bees have been reported to collect propolis material. Important sources include poplars, alders and birches, chestnut, ash, various Prunus and willows. Variations in the beeswax content of raw propolis also affect the chemical composition.
Studies indicate that the plant resins collected by bees are at least partially altered by bees prior to use in the hive.
5. Human Nutrition
Propolis has little direct nutritive value, apart from the presence of small amounts of proteins, amino acids, minerals and sugars. Vitamins include small amounts of A, B1, B2, B6, C and E (Ghisalberti, 1979).
Dihydroflavonoids, like those found in propolis, have been shown to aid the human body in absorbing Vitamin C (Bors, et al, 1995).
Propolis is used by humans almost solely as a therapeutic. Propolis and a number of its components exhibit a wide variety of biological and pharmacological activities (Schmidt and Buchmann, 1992).
6. Therapeutic Properties
6.1 Antimicrobial Effect
Because of its strong antimicrobial activity, propolis is often known as a "natural antibiotic". A large number of studies have shown an inhibitory effect on a variety of micro-organisms, The antimicrobial effects are summarized in the following table (from Krell, 1996 and others) :
|Bacillus larvae||destroyed||Mlagan and Sulimanovic, 1982|
|B. subtilis||destroyed||Meresta and Meresta, 1985|
|Helicobacter pylori||inhibited||Itoh, et al, 1994|
|MRSA||strong inhibition||Grange and Davey, 1990|
Grange and Davey, 1990
|Staphylococcus sp.||inhibited||Chernyak, 1973|
|Staphylococcus aureus||synergistic effect||Kedzia and Holderna, 1986|
|Streptococcus sp.||inhibited||Rojas and Cuetara, 1990|
|Streptomyces||inhibited||Simuth et al, 1986|
|S. sobrinus, mutans, cricetus||dental caries||Ikeno et al, 1991|
|Saccharomyces cerevisiae||brewer’s yeast||Petri et al, 1988|
|Escherichia coli||inhibited||Simuth et al, 1986|
|Salmonella||potential treatment||Okonenko, 1986 and others|
|Giardia lambia||positive effect||Olarin et al, 1989 and others|
|Bacteroides nodosus||reduced foot rot||Munoz, 1989|
|Klebsiella pneumoniae||positive effect||Dimov et al, 1991|
|Candida albicans||synergistic effect||
Holderna and Kedzia, 1987
|Aspergillus niger||positive effect||Petri et al, 1988|
|Botrytis cinerea||in vitro fungicidal||La Torre et al, 1990|
|Ascosphaera apis||inhibited||Ross, 1990|
|Herpes||inhibited in vitro||Sosnowski, 1984|
|Potato virus||effective||Fahmy and Omar, 1989|
|Influenza (in mice)||reduced mortality||Serkedjieva, 1992 and others|
|Newcastle disease||affected virus
|Maksimova-Todorova et al, 1985|
Active components of propolis showing an antibacterial effect include pinocembrin, galangin, caffeic acid and ferulic acid. Antifungal components include pinocembrin, pinobanksin, caffeic acid. benzy ester, sakuranetin and pterostilbene. Anti-viral components include caffeic acid, lutseolin and quercetin (Schmidt and Buchmann, 1992). Propolis has been found to inhibit the synthesis of protein by bacteria, which may account for at least some of Its antimicrobial effects (Simuth, et al, 1988).
6.2 Synergistic Effects
Most studies on the therapeutic properties of propolis have centered on the phenolic constituents (flavonoids and other phenolic compounds such as caffeic acid esters). Research has tended to isolate and test single substances in propolis. However, it is likely that the presence of a large number of compounds in propolis may produce a syngeristic effect greater than the sum of the effects of individual components (Houghton, 1998). Studies have shown that the flavonoids in propolis exert significant antibacterial activity, but that isolated flavonoids show reduced activity compared to whole product extracts (Bone. 1996). 2 Propolis has also been shown to have a synergistic effect with certain antibiotics, and to increase their effectiveness on some bacteria and yeasts some cases 100 fold (Kivalina and Gorshunova, 1973). Antibiotic-resistant strains of Staphylococcus were found to become sensitive to antibiotics in combination with propolis (Shub, 1981).
6.3 Anticancer Effects
Ethanol extracts of propolis have been found to transform human hepatic and uterine carcinoma cells In vitro, and to inhibit their growth (Matsuno, 1992). Substances isolated in propolis which produce this cytotoxic effect are quercetin, caffeic acid, and clerodane diterpendoid. Clerodane diterpendoid shows a selective toxicity to tumour cells.
Propolis was also found to have a cytotoxic and cytostatic effect in vitro against hamster ovary cancer cells and sarcoma-type tumours in mice (Ross, 1990). The substance has also displayed cytotoxicity on cultures of human and animal tumour cells, including breast carcinoma, melanoma, colon, and renal carcinoma cell lines. (Grunberger et al, 1988). The component producing these effects was identified as caffeic acid phenethy ester.
A substance called Artepillin C has been isolated from propolis, and has been shown to have a cytotoxic effect on human gastric carcinoma cells, human lung cancer cells and mouse colon carcinoma cells in vitro (Kimoto, et al, 1995).
Caffeic acid esters have been shown to inhibit chemically induced tumour production in mice, as well as having a selective toxic effect on cells affected by genes which promote the development of cancerous cells (Su, et al, 1996).
6.4 Antioxidant Effects
The flavonoids concentrated in propolis are powerful antioxidants. Antioxidants have been shown to be capable of scavenging free radicals and thereby protecting lipids and other compounds such as Vitamin C from being oxidized or destroyed (Popeskovic, et al. 1980).
It is probable that active free radicals, together with other factors. are responsible for cellular aging and degradation in such conditions as cardiovascular diseases, arthritis, cancer, diabetes, Parkinson disease and Alzheimer disease. Oxidatve damage may also result in poor liver function. Studies on rats in vitro show that propolis extracts protect against damage to liver cells (Baset, et al, 1996).
6.5 Wound Healing and Tissue Repair EffectsPropolis has been shown to stimulate various enzyme systems, cell metabolism, circulation and collagen formation, as well as improve the healing of burn wounds (Ghisalberti, 1979; Krell, 1996). These effects have been shown to be the result of the presence of arginine in propolis (Gabrys, et al, 1986). Propolis and aloe vera was found to be superior to standard wound treatment products in trials on mice (Sumano-Lopez, et al, 1989).
6.6 Gastro-Intestinal Effects
Propolis has been shown to inhibit the development of externally induced stomach ulcers in rats (Aripov, 1988). Flavonoid components of propolis have also been shown to have this effect (Ciaceri and Attaguile, 1972).
6.7 Skin Infection Effects
Propolis has been shown to be effective in inhibiting the growth of yeasts and fungi responsible for such skin infections as ringworm and athlete's foot (Metzner, et al, i979). Propolis compounds showing activity against these organisms are the flavonoids and caffeic acid derivatives.
6.8 Anti-Inflammatory Effects
Studies on mice have shown that extracts of propolis have an anti-inflammatory effect similar to that of indomethacin, a common drug used to treat inflammation. Again, flavonoids and caffeic acid are known to play a role in inhibiting the inflammatory response (Mirozeva and Calder, 1996).
6.9 Anaesthetic Effects
Propolis and some of its components produce anaesthesia, which in some studies has been shown to be 3 times as powerful as cocaine and 52 times that of procaine, when tested in rabbit cornea (Ghisalberti, 1979). The anaesthetic effect has been shown to be produced by pinocembrin, pinostrobin, caffeic acid esters components in propolis (Paintz and Metzner, 1979).
The anaesthetic effect may explain why propolis has been used for centuries in the treatment of sore throats and mouth sores. An anaesthetising ointment for dentistry using propolis has been patented in Europe (Sosnowski, 1984).
6.10 Effects on Immune System
Propolis has been shown to stimulate an immune response in mice (Manolova, et al, 1987). More recently, Japanese researchers have shown an extract of propolis to produce a macrophage activation phenomenon related to the immune function in humans (Moriyasu, et al, 1993). Propolis activates immune cells which produce cytokines. The results help to explain the ant-tumour effect produced by propolis.
Propolis has been shown to stimulate antibody formation in immunized mice. In a joint US-Polish study, spleen cells producing antibodies in mice administered a propolis extract were three times greater than controls. A second dose administered 24 hours later produced an even larger effect, although further doses reduced the effect (Scheller, et al, 1988).
Propolis was shown to increase antibody formation between 2-3 times that of controls in pigs vaccinated with "BUK-628" live Aujeszky's disease vaccine with and without addition of propolis. Antibody formation reached its maximum in 14 days, and antibodies could be detected for up to 330 days. Propolis also enhanced production of plasmacytes in the lympholdal tissue of the spleen and lymph nodes (Karandashov, et al, 1977). Propolis has been shown to suppress HIV-1 replication and modulate in vitro immune responses, and, according to the authors, "May constitute a non-toxic natural product with both anti-HIV-1, and immunoregulatory effects" (Harish, et al, 1997).
6.11 Cardiovascular Effects
In mice a concentrated extract of propolis has been shown to reduce blood pressure, produce a sedative effect, and maintain serum glucose (Kedzia et a11 1988). Dihydroflavonoids1 as contained in propolis, have been shown to strengthen capillaries (Roger, 1988), and produce antihyperlipidemic activity (Chol, 1991). Propolis has also been shown to protect the liver against alcohol (ethanol) and tetrachloride in rats (Coprean,et al, 1986).
6.12 Dental Care Effects
In rats inoculated with & sobrinus, about half of their fissures were carious, while dental canes were significantly less in rats given water containing propolis extracy. No toxic effects of propolis on the growth of rats were observed under experimental conditions in this study (Ikeno, et al, 1991). Propolis has also been shown to be effective as a subsidiary treatment for gingivitis (gum infections) and plaque (Neumann, et al, 1986). A 50% propolis extract was found to antiseptic against pulp gangrene (Gafar, et al, 1986). Propolis has also been shown to inhibit the growth of a range of bacterial organisms found in dental caries (Ota, et al, 1996).
7. Clinical Effects on Humans
The diverse use of propolis In clinical trials shows that its therapeutic efficiency lies mainly in diseases caused by microbial contamination (Marcucci, 1995).
7.1 Respiratory Infections
A total of 260 steel workers suffering from bronchitis were treated for 24 days by various methods including local and systemic regulation of the Immune system and local treatment with an ethanol extract of propolis (EEP) in a physiological salt solution. Best results were obtained with inhalation of the extract, together with propolis tablets (Scheller et al, 1989a).
Propolis has also shown positive effects In other otorhinolaryngologic diseases, such as pharyngitis (Doroshenko, 1975), chronic bronchitis (Scheller, et al, 1989a), rhinopharyngolaryngitis (Isakbaev, 1986), pharyngolaryngitis (Lin, et al, 1993), catarrh (Zommer-Urbanska et al, 1987), and rhinitis (Nunex. et al, 1938).
7.2 Viral Infections
A clinical trial has shown a prophylactic effect against influenza infection in humans (Vosnjak, 1978). Mother clinical trial showed that infections of the common cold were shorter in duration and completely recovered within 3 days for patients treated with propolis, compared to 5 days for recovery for patients not given propolis. (Scheller et al, 1989).
A clinical trial conducted on dermatology patients showed a propolis cream had significant therapeutic effects against recurrent herpes (Herpes simplex Type 1) and Herpes zona zoster (shingles). The propolis cream reduced duration of lesions and pain, and increased interval between lesion episodes (Giurcaneanu, et al, 1988).
7.3 Skin InfectIons
Clinical applications of propolis (1-10%) in ether or alcohol were effective against 10 superficial fungi and 9 deep-growing fungi. On oral treatment of 160 psoriasis patients with 0.3g propolis 3 times daily for 3 months, about one-third were cured or greatly improved (Fang Chu, 1978). Patients (110) Infected with ringworm were treated with 50% propolis as a unguent. In 97 patients it was f ound to produce excellent results (Bolshakova, 1975).
7.4 Dental Treatment
Sixty students were divided into groups to test the effect of propolis on the development of plaque and gingivitis. The results suggest that a propolis preparation can be a useful subsidiary treatment in oral hygiene (Neumann et al, 1988).
A double-blind clinical trial showed that a propolis mouthwash (10% tincture diluted l:5 with water) produced significant improvements In patients with gingivitis and periodontal disease. Patients were evaluated for plaque formation and inflammation of the gums (Schmidt. et al, 1980). A clinical study used a paste made from propolis extract and zinc oxide on 150 teeth with indirect capping of deep cavities, and 50 teeth with direct capping. The results showed that the paste with propolis exerted effects similar to those of zinc eugenate, and were superior for healing compared to pastes based on calcium hydroxide (lonita, et a1, 1990). A clinical study found propolis useful for the treatment of gum inflammation and oral mucosa, and also showed anti scarring effects (Gafar, et all 989). Another study showed similar results for periodontitis and suggested propolis be used in root canal fillings because of its bone-regeneration and anaesthetic properties (Kosenko and Kosotch, 1990).
7.5 Wound Healing and Tissue Repair
Sixty four patients with tibial skin ulcers, aged from 23 to 98 years, were treated using propolis tincture in an ointment. The ointment was applied daily to the ulcerated area, which was also treated on the periphery with antibiotic ointments. The treatment lasted for 4-12 weeks. At the end of treatment, 19 of the 84 treated patients exhibited no clinical signs of the condition, 19 an improved condition (Korsun, 1983). Propolis was used in a trial of hospital patients with infected wounds. The propolis improved wound healing rates, while at the same time reducing infection. Over half of infective bacteria were eliminated within 4 days. Propolis did not produce antibio~resistance strains of the bacteria (Damyanliev, 1982).
A study of topical application of propolis on wounds, burns and ulcers showed up to an 80% increase in healing rate compared to controls using routine healing regimes (Scheller, 1980). Patients (229) with burns, clean wounds, infected wounds or abscesses / ulcers were treated with cream containing propolis at two concentrations (2% and 8%). The higher concentration caused local intolerance in 18% of patients by day 9, whereas the lower concentration caused symptoms in only 1.8% of patients by day 16. Burns and wounds treated with the low concentration cream healed in 11 days on average, septic wounds in 17.5 days, 67% of ulcers in 38 days (Morales and Garbarino, 1996).
7.6 Ear Infections
Patients (126) suffering external otitis, chronic mesotypanic otitis and tympan perforation were treated with propolis solutions (5-10%). A positive therapeutic result was reported in most cases (Matel, et al, 1973). Propolis has also shown positive results in the treatment of acute inflammations of the ear(Palos, et al, 1989).
7.7 Gastro-Intestinal Problems
Patients (138) suffering giardiasis were treated with propolis extracts (10-20%). In children, 52% showed a cure at the lower dose. In adults, the cure rate was the same as for tinidazole an antiprotozoan drug, at the 20% extract, and 60% versus 40% for Undazole at a higher concentration (30% propolis extract) (Mirayes, et a11 1988).
Propolis was used to treat ulcerative colitis and Crohn's disease in a double-blind clinical trial in Denmark. Improvement was noted in patients with colitis, but no effect was shown against Crohn's disease (Stolko, et al. 1978).
7.8 Immune Deficiency
A strong immune deficiency was found in 2 patients with alveolitis fibroticans. Treatment with a combination of propolis, Esberitox N and calcium-magnesium resulted in good improvements in the state of the immune system and the clinical condition of both patients (Scheller et al, 1989 b).
4 Injections of an aqueous solution of propolis were used in the treatment of 22 patients with this hip joint disease caused by aseptic necrosis of the thigh bone. A further 32 patients with the same condition were given different forms of routine treatment. Significant improvement was observed In the patients given propolis (Przybylski and Scheller, 1985).
Patients (90) with cases of vagina and uterus cervix inflammation caused by S. pyogenes were treated with 3% propolis ethanol extract. Over 50% of the cases responded well to this treatment (Zawadzki and Scheller, 1973).
8. Adverse Effects
Propolis has been shown not to be toxic to humans or mammals unless very large quantities are administered (Ghisalberti, 1979). Some of its constituent flavones, eg., quercetin, might be mutagenic by the Ames test, but mutagenicity per SE for propolis has not been reported (Schmidt and Buchmann, 1992).
Contact dermatitis is a well documented allergic reaction to propolis, with appoximately 200 cases reported in the literature over the last 70 years (Hausen et al, 1987). Initial reports were made by beekeepers, who came into daily contact with the raw product. Allergic reactions are now also reported in the general population, due to the more wide-spread use of products containing propolis.
Dermatitis can be produced by skin contact with raw propolis, as well as propolis extracts and products containing caffeic acid and its derivatives have been identified as the major allergenic agent (Hashimoto, et al, 1988). Cinnamic acid derivatives have also been implicated (Scheller and Frosch, 1988). Dermatitis is relieved once the skin is no longer in contact with the propolis product. It is therefore recommended that with all preparations intended for human use, usage is ceased whenever there is an allergic reaction. Very few other adverse reactions to propolis have been documented in the literature, and the product is considered generally not to be harmful (Schmidt and Buchmann, 1992). Rare cases of oral inflammation and ulceration, mouth oedema (swelling) and stomatitis have been reported. however, as a result of oral ingestion of propolis (Hay and Grieg, 1990; Wanscher, 1978).
9. Commercial Use
Raw propolis is collected by beekeepers and sold in bulk to companies that refine the product and turn it into usable extracts. Main commercial uses of propolis are as a dietary supplement and therapeutic. Propolis is sold in tablets (singularly, or in combination with other substances such as pollen, royal jelly and non-hive products). In Japan, the use of propolis is permitted as a preservative in frozen fish (Irrell, 1996).
Tinctures and lozenges are popular treatment for sore throats, and tinctures are often used to treat Cuts, mouth sores and skin rashes. For internal use, 1-3mL does three times daily of a 1:10 tincture are typical, but higher doses can use used if necessary. Propolis tincture is normally diluted in water. producing a cloudy liquid. For external use, the 1:10 tincture is diluted in water, and used as a lotion or gargle (Bone, 1996).
Propolis is a stable product. but should nevertheless be stored in airtight containers in the dark, preferably away from excessive and direct heat. Propolis does not lose much of its antibiotic activity, even when stored for 12 months or longer. Propolis and its extract function as a mild preservative due to their antioxidant and antimicrobial activities and thus may actually prolong the shelf life of some products (Irell, 1996).
Because of its antioxidant and antimicrobial activities, microbial contamination is not considered to be a problem with propolis, either in the raw form, or as extracts. Concentrations of lead above maximum allowable levels for food products have been found in propol is. studies have shown that lead levels may be reduced by placement of hives away from areas with heavy air pollution and the use of oil based paints on hive parts (Alcici, 1996). Propolis destined for commercial use should be routinely tested for lead concentration. Brazilian propolis is of the highest quality available where Chinese propolis has been noted for excessive lead.
No international standard exist for propolis. Official standards exist for propolis in several East European countries. Japanese standards for Propels offer some of the highest none official standards.
12. Bee Propolis - Past to Present
Propolis has been used by man for thousands of years and recently has enjoyed a boom in popularity. Bees have used propolis for millions of years, and humans have used it for thousands. Both species find it immensely useful and beneficial. Much of the bees' success in surviving through the ages may be accredited to propolis. As humans, we may yet discover we've only just scratched the surface to the benefits of this resinous wonder.
The Greek physician, Hippocrates, prescribed the use of propolis to help heal internal and external sores and ulcers. Ancient Egyptians depicted propolis-making bees on vases and other ornaments, and used the resinous substance to alleviate many ailments. Pliny, the Roman scholar, wrote much on the use of resins such as propolis in his massive book, Natural History. He touts the abilities of propolis to reduce swelling, soothe pain, and heal sores, to name a few.
In The History of Plants written by John Gerard in 1597, propolis was noted for its ability to provide swift and effective healing for many conditions. During this era, propolis was used in many different healing ointments.
Propolis is a sticky resin which seeps from the buds of certain trees and oozes from the bark of other trees. Although propolis is vitally important to the colony, there are usually just a few propolis gathering specialists in the hive. The bees gather propolis, sometimes called "bee glue," and carry it home in their pollen baskets. There they are met by one or two other worker bees who help them unload. These workers take the resinous material and add salivary secretions and wax flakes to it, then use the new product for numerous protective purposes as bee propolis. The bees use it to coat the inside of the hive, including the passageway and the brood chambers.
Propolis protects the hive in two ways: First, it reinforces the hive itself; second, it protects the hive from bacterial and viral infection. And it is these latter properties which man has found so helpful through the centuries. The reason propolis is such an effective protector is related to the diversity of flavonoids. Propolis consists of approximately 55 percent resinous compounds and balsams, 30 percent beeswax, 10 percent aromatic oils, and 5 percent bee pollen. Other constituents include flavonoids, amino acids, B vitamins, and most importantly, antibiotic substances. Often called "nature's penicillin, " bee propolis has effective antibacterial, antiviral, antiseptic, antifungal, and antibiotic-properties. These protective and healing properties have been conclusively demonstrated in numerous studies all over the globe.
In the former Soviet Union, V.H. Karinova and E.l. Rodionova conducted a study on 135 patients suffering from various forms and stages of tuberculosis. Their patients' ages ranged from six to 50. Patients were given bee propolis three times daily for four to 10 months depending upon response to treatment. By the end of the study, all but 12 of the patients had improved dramatically, including some patients going into regression. The12 who did not respond favorably all suffered from kidney tuberculosis.
In Romania, Drs. A. Vasilca and Eugenia Milcu conducted a study on the therapeutic properties of propolis on ulcers. Thirty four patients with chronic ulcers were given extracts of propolis daily for two weeks. The results were impressive, with 28 patients completely recovering and six cases dramatically improving. Tissue biopsies were conducted on some of the patients, which confirmed the regenerative effects of bee propolis.
Medical researchers N. Popovic and N. Oita of Rumania published a report on the effects bee propolis has on mitosis (the process of cell division). They reported that a tissue never becomes entirely malignant; it always contains some normal cells, but the activity of the normal cells is affected and even repressed by malignant cells. Bee propolis favors the activity of normal cells by repressing malignant cells, which helps the tissue to reestablish its normal condition. Constituents of propolis have a mitodepressive effect (depression of the proliferation of cancerous cells) on cells deranged by malignancy.
In Bulgaria, Dr. S. Nikolov, et al, investigated the efficacy of bee propolis in the treatment of acute and chronic colitis. Forty five patients, both men and women aged 20 to 65 years old and suffering from either acute or chronic colitis, took part in the study. They were given extracts of bee propolis three times a day before meals. In 43 of the patients results were positive, with 26 showing very good response, 12 showing good response, and five showing satisfactory. Only two patients showed no improvement. In most cases, pain began to diminish in seven days, disappearing on the nineteenth or twentieth day.
Perhaps the most broadly investigated and widely accepted attribute of bee propolis is its immune-boosting activity. It is a natural, broad-spectrum antibiotic that activates the thymus gland. Bee propolis not only prevents infectious diseases, but clears them from the system, as well. As demonstrated in numerous experiments, propolis has the ability to directly destroy bacteria, viruses, and fungi, even penicillin-resistant staphylococcus. Bee propolis is formidable against viruses. This trait is attributed to the bioflavonoids present in propolis, which have a protective effect against viral infections. Viruses are enclosed in a protein coating. As long as it remains unbroken, the infectious and dangerous material remains imprisoned and is harmless to the host organism. Unfortunately, within the host their are enzymes which remove the protein coating, thus releasing the harmful material to wreak havoc within the system. With the presence of bee propolis in the system, however, this doesn't occur. The bioflavonoids inhibit the enzymes from removing the protein coating, keeping the viral material locked inside. These same flavonoids maintain the protective coating around the virus, thus rendering it inactive. With the presence of the bioflavonoids, the host virtually becomes immune to the virus. Another way in which propolis aids the immune system is its ability to strengthen phagocyte activity. Phagocytes are cells that are able to surround, engulf, and digest microorganisms and cellular debris. This increase in activity with the introduction of bee propolis was observed and documented by a number of Soviet and European scientists.
The Power of Propolis
The power of propolis is wide-ranging and of immense benefit to humans, as well as to its creator-the little honey bee. People suffering from high levels of blood fat can benefit from taking bee propolis. At the Worker's Hospital of Lian Yun Gang, Jiangsu Province in the People's Republic of China, Dr. Fang Zhu chose 45 patients suffering from hypertension, arteriosclerosis, and coronary heart disease and gave them 300 mg of bee propolis three times a day for 30 days. At the end of that period all patients showed a significant reduction of blood fats and improvement in related disorders Another benefit of propolis is its inhibitory effect on certain prostaglandins, which it accomplishes by blocking the enzymes that form specific prostaglandins. This can be of immense benefit to those suffering from aches and fever, which are caused by prostaglandins. Bee propolis acts in nearly the identical way aspirin does by blocking the same enzymes, yet without the negative side effects you can get with aspirin. This enzyme-blocking, prostaglandin-inhibitory effect is also beneficial to the mouth and throat. For instance, a leading cause of dental problems is the erosion of the gums and tissues that line the tooth sockets. Inflammation and infectious bleeding can cause a weakening of the bone structure and tooth loss. But propolis, by blocking specific enzymes, prohibits the formation of the prostalandins which cause the inflammation, bleeding, and eventual decomposition. At the same time, propolis actually stimulates other specific enzymes which strengthen the walls of the blood vessels in the gums, thereby having a twofold effect on the mouth. When inflamed and sore, the throat responds favorably to propolis, and for the same prostaglandin inhibition reasons. By inhibiting prostaglandin formation, inflammation recedes and diminishes.
Another attribute of bee propolis is its ability to correct and stabilize proper protein metabolism. A team of physicians at the Institute of Radiology at Serajevo, Yugoslavia treated patients who were suffering from radiation complications. These patients had serious liver damage caused by improper protein metabolism and X-rays. The patients were given bee propolis for two months. Another group of patients, also suffering from radiation complications, were given a placebo. At the end of two months, those taking bee propolis had significantly improved, with some patients' symptoms completely disappearing. No improvements were observed in the group given the placebo.